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Articles

Vol. 1 No. 3 (2010): July–September 2010

Docking Studies on Transcription Factor sp1: The Transcriptional Down-Regulation of TACO Gene by EGCG and the Importance of TACO in M. tuberculosis Survival

Submitted
November 24, 2024
Published
2010-08-15

Abstract

Host molecule tryptophan-aspartate containing coat protein (TACO) helps both in entry as well as intracellular survival of M. tuberculosis. Transcription factor SP1 is a potential candidate which regulates expression of TACO protein (3). Sp1 binding region is present within TACO gene promoter. Green tea polyphenols like Epigallocatechin gallate and vitamin-D, retinoic acid, has the inherent capacity to downregulate TACO gene transcription in a dose-dependent fashion. A series of experimentally tested vitamins and polyphenols has been docked to the active site of the Transcription factor SP1 using AutoDock 4.2 program. The free energies of binding (∆G) and inhibition constants (Ki) of the docked compounds were calculated by the Lamarckian Genetic Algorithm (LGA). These values suggest that EGCG and theaflavin are excellent inhibitors of SP1, and down regulate TACO protein. EGCG may be useful as an effective adjuvant therapy against tuberculosis.

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